Journal: The Journal of Neuroscience

Article Title: Dorsal–Caudal and Ventral Hippocampi Target Different Cell Populations in the Medial Frontal Cortex in Rodents

doi: 10.1523/JNEUROSCI.0217-25.2025

Figure Lengend Snippet: Anterograde transsynaptic spread of AAV1-Cre injections from dcHPC and vHPC target superficial and deep layers of MFC in mice. A , AAV1-hSyn-Cre was injected in either dcHPC or vHPC resulting in expression in HPC projection neurons (data not shown). The transsynaptic spread to postsynaptic neurons was detected by immunostaining against Cre. Note that postsynaptic neurons likely include principal neurons and interneurons in MFC which, in the scheme, are represented by triangles and circles, respectively. The present experiments do not include a further differentiation between interneurons and principal neurons. B , Summary of AAV1-Cre injection sites along HPC displayed in coronal sections. Injection sites in dcHPC and vHPC are shown in cool and warm colors, respectively. C , Representative samples of MFC coronal sections showing the distribution of postsynaptic Cre-expressing neurons in MFC for dcHPC injections (left, case #567) and high-magnification image of the boxed region (right). Scale bars, 500 µm. D , Representative samples of MFC coronal sections showing the distribution of postsynaptic Cre-expressing neurons in MFC for vHPC injections (left, case #565) and high-magnification image of boxed region (right). Scale bars, 500 µm. E , Representative scheme showing the distribution of postsynaptic Cre-labeled neurons across the anterior–posterior axis of MFC for one dcHPC (case #567, green) and one vHPC (case #565, magenta) injection. F , Proportion of the number of Cre-labeled cells in a MFC subregion among the total Cre-labeled neurons across MFC sections for dcHPC (green, n = 5) and vHPC (magenta, n = 5) injections. Data are presented as violin plots, with each circle corresponding to one sample. Values from all subregions sum up to 1 for one individual sample. Mann–Whitney test corrected for multiple comparisons using the Holm–Šídák method: * p < 0.05. G , H , Laminar distribution of postsynaptic Cre-labeled cells in superficial and deep layers of MFC subregions for dcHPC ( E ) and vHPC ( F ) injections. Data are presented as violin plots, with each circle corresponding to one sample. Values from superficial and deep layers of all subregions sum up to 1 for one individual sample. Mann–Whitney test corrected for multiple comparisons using the Holm–Šídák method: * p < 0.05.

Article Snippet: For the anterograde transsynaptic experiments, AAV1-hSyn-Cre (1.9 × 10 13 to 2.1 × 10 13 GC/ml; 200 nl; Addgene-10553) was injected in either dcHPC or vHPC, and either AAV8-hSyn-DIO-mCherry (3.6 × 10 12 GC/ml; 3 × 100 nl; Addgene-50459) or AAV9-hDlx-Flex-GFP (4.4 × 10 12 GC/ml; 3 × 100 nl; Addgene-83895) was injected along the dorsoventral axis of MFC.

Techniques: Injection, Expressing, Immunostaining, Labeling, MANN-WHITNEY

Journal: The Journal of Neuroscience

Article Title: Dorsal–Caudal and Ventral Hippocampi Target Different Cell Populations in the Medial Frontal Cortex in Rodents

doi: 10.1523/JNEUROSCI.0217-25.2025

Figure Lengend Snippet: dcHPC and vHPC inputs to MFC innervate GABAergic and non-GABAergic neurons in mice. A , AAV1-hSyn-Cre was injected in either dcHPC or vHPC and was expressed in HPC projection neurons. The transsynaptic spread led to Cre expression in postsynaptic neurons, including principal neurons and interneurons in MFC. In parallel, AAV8-hSyn-DIO-mCherry was injected in MFC leading to Cre-dependent mCherry expression in postsynaptic neurons in MFC. Postsynaptic interneurons were subsequently differentiated by immunostaining against GAD67, as illustrated in D–F . In the scheme, principal neurons and interneurons are represented by triangles and circles, respectively. B , Summary of AAV1-hSyn-Cre injection sites along the HPC displayed in coronal sections. Injection sites in the dcHPC and vHPC are shown in cool and warm colors, respectively. C , Representative samples with AAV1-hSyn-Cre injection in either dcHPC (left, case #611) or vHPC (right, case #696). Images show the postsynaptic Cre-dependent mCherry expression in a coronal section of MFC. Scale bar, 500 µm. D , Representative sample showing the mCherry expression of postsynaptic neurons and colocalization with the GAD67 antibody labeling as used to identify the GABAergic neurons among the HPC-MFC postsynaptic cells. Scale bar, 20 µm. E , Proportion of cells coexpressing mCherry and GAD67 among the total mCherry+ neurons receiving dcHPC (green, n = 6) or vHPC (pink, n = 6) inputs in MFC. The proportions were compared with the native presence of GAD67 in MFC (light-blue, n = 4). One-way ANOVA, followed by post hoc Tukey's multiple-comparison test: *** p < 0.001; **** p < 0.0001. F , Subregional distribution of GABAergic (GAD+) and non-GABAergic (GAD−) neurons in MFC receiving inputs from dcHPC (left, n = 6, green tones) and vHPC (right, n = 6, pink tones) Cre injections. The proportions of inputs to GAD+ and GAD− cells across MFC subregions sum up to 1 for each injection sample. Data are presented as violin plots, where each circle corresponds to one sample. Two-tailed t tests: ** p < 0.01; * p < 0.05.

Article Snippet: For the anterograde transsynaptic experiments, AAV1-hSyn-Cre (1.9 × 10 13 to 2.1 × 10 13 GC/ml; 200 nl; Addgene-10553) was injected in either dcHPC or vHPC, and either AAV8-hSyn-DIO-mCherry (3.6 × 10 12 GC/ml; 3 × 100 nl; Addgene-50459) or AAV9-hDlx-Flex-GFP (4.4 × 10 12 GC/ml; 3 × 100 nl; Addgene-83895) was injected along the dorsoventral axis of MFC.

Techniques: Injection, Expressing, Immunostaining, Antibody Labeling, Comparison, Two Tailed Test

Journal: The Journal of Neuroscience

Article Title: Dorsal–Caudal and Ventral Hippocampi Target Different Cell Populations in the Medial Frontal Cortex in Rodents

doi: 10.1523/JNEUROSCI.0217-25.2025

Figure Lengend Snippet: dcHPC and vHPC innervate both PV and SOM interneurons in the mouse MFC. A , Representative images showing the native distribution of the indicated marker expression (left, PV; right, SOM) in the subregions of MFC. Scale bar, 500 µm. B , AAV1-hSyn-Cre was injected in either dcHPC or vHPC and expressed in HPC projection neurons. The transsynaptic spread led to Cre expression in postsynaptic neurons, including principal neurons and interneurons in MFC. In parallel, AAV9-hDlx-Flex-GFP was injected in MFC, leading to Cre-dependent GFP expression in postsynaptic interneurons in MFC. In the scheme, principal neurons and interneurons are represented by triangles and circles, respectively. C , Summary of AAV1-hSyn-Cre injection sites along HPC in coronal sections. Injection sites in dcHPC and vHPC are shown in cool and warm colors, respectively. D , Micrographs showing the viral GFP expression of labeled GABAergic neurons and colocalization with the GAD67 antibody. Scale bar, 20 µm. E , Micrographs showing the viral GFP expression and colocalization of GFP+ cells and the indicated markers (PV+ in red, SOM+ in violet). Scale bar, 100 µm. F , Representative schemes showing the distribution of the viral GFP-expressing interneurons in MFC receiving inputs from dcHPC (left, case #773) and vHPC (right, case #772) Cre injections that coexpress either PV or SOM across the MFC subregions. G , Pie charts showing the proportion of GFP+ cells coexpressing PV (red) or SOM (violet) among the total GFP-labeled interneurons receiving dcHPC (left, n = 6) or vHPC (right, n = 4) inputs. H , I , Subregional distribution of the GFP+ interneurons receiving HPC inputs in MFC that coexpress the indicated markers PV (red) or SOM (violet) for dcHPC ( H , n = 6) and vHPC ( I , n = 4). Data are presented as violin plots, with each circle corresponding to one sample. For one sample, proportions of PV+ and SOM+ cells across the MFC subregions do not sum up to 1, with the residual proportion corresponding to unidentified GFP+ interneurons. A Mann–Whitney test corrected for multiple comparisons using the Holm–Šídák method.

Article Snippet: For the anterograde transsynaptic experiments, AAV1-hSyn-Cre (1.9 × 10 13 to 2.1 × 10 13 GC/ml; 200 nl; Addgene-10553) was injected in either dcHPC or vHPC, and either AAV8-hSyn-DIO-mCherry (3.6 × 10 12 GC/ml; 3 × 100 nl; Addgene-50459) or AAV9-hDlx-Flex-GFP (4.4 × 10 12 GC/ml; 3 × 100 nl; Addgene-83895) was injected along the dorsoventral axis of MFC.

Techniques: Marker, Expressing, Injection, Labeling, MANN-WHITNEY

Journal: eNeuro

Article Title: FKBP51 in the Oval Bed Nucleus of the Stria Terminalis Regulates Anxiety-Like Behavior

doi: 10.1523/ENEURO.0425-21.2021

Figure Lengend Snippet: Fkbp5+ cells in the ovBNST coexpress with the neuropeptides Crh and Tac2 and their number is significantly increased after exposure to ASR. A , Fkbp5 and Tac2 are coexpressed in the ovBNST, as can be seen in detail (violet outline arrow). Fkbp5 and Crh are also coexpressed in the ovBNST as shown in detail (violet arrow). B , Expression patterns of Fkbp5 with Tac2 and Crh in the ovBNST also strongly overlapped (gray outline arrow). In addition, there were some cells that expressed Fkbp5 only (gray arrow). C , Quantification of the number of cells expressing Fkbp5 only, coexpressing Fkbp5 and Tac2 , coexpressing Fkbp5 and Crh , and coexpressing Fkbp5 , Tac2 , and Crh after exposure to ASR resulted in significant upregulation across all cell types. Scale bar ( A , B ): 25 μm. Data are mean ± SEM; * p < 0.05, ** p < 0.01.

Article Snippet: Manipulation of FKBP51 was performed using adeno-associated bicistronic AAV1/2 vectors (AAV1/2-HA-CAG-FKBP5-1 and AAV1/2-CAG-null, GeneDetect; AAV1-CMV-Cre and AAV1/2-CAG-empty-IRES-EGFP, Addgene; AAV1/2-ESARE-ER T2 CreER T2 ).

Techniques: Expressing